Capillaries Muscle: Normal Endoneurial Microvessels Capillary pathology Microvasculopathy C5b-9 Deposits HIEM (Nerve) Muscle Enlargement Pipestem SRP Myopathy TS-HDS neuropathy Hemosiderin Endoneurial inflammation Ipilimumab Perineuritis Loss DM-VP GvHD Systemic Sclerosis Minimal myopathy Ultrastructure Myopathy + Capillary Δ Subperineurial edema Thick vessel walls ELS vessels |
![]() Endomysial Capillary: Normal
|
![]() |
Basement membrane Around & outside endothelial cell layer May be homogeneous or layered Contains pericytes (P) Composition Collagen IV Laminin Glycoproteins: Fibronectin, Nidogen-1, Nidogen-2 (Entactin) Proteoglycans (as HSPG): Perlecan, Agrin Thickening: Differential diagnosis |
Endothelial cells (E) Surround lumen Stains: Ulex Europaeus Agglutinin I (UEAI); CD31 Pericytes (P) Location: Within basal lamina of vessel wall Contractile Frequency vs Endothelial cells: 1:10 to 1:100 Stain: PDGRFβ Smooth muscle Stain: Smooth muscle actin |
![]() UEA I stain |
![]() UEA I stain |
![]() UEA I stain |
![]() UEA I stain |
![]() UEA I stain |
![]() UEA I stain |
![]() UEA I stain |
![]() UEA I stain |
![]() UEA I stain |
![]() UEA I stain |
![]() H&E stain Endoneurial Microvessel (Arrow) Orientation: Along length of nerve |
![]() Toluidine blue stain |
![]() |
![]() Ultrastructure: From Robert E Schmidt MD |
![]() Ultrastructure: From Robert E Schmidt MD |
Diabetes Endoneurial vessel in Nerve Basal lamina: Multiple layers |
Non-diagnostic muscle Endomysial vessel in Muscle Thickened wall: Homogeneous |
![]() From: Chunyu Cai |
![]() |
![]() |
![]() |
Basal lamina of Endoneurial microvessels
Thickened; Homogeneous (Arrows) |
Basal lamina of Endoneurial microvessel Normal (Arrow) |
![]() |
![]() |
![]() |
![]() |
Microvessels C5b-9 deposition on Microvessels C5b-9 around Endotheial cells Endoneurial Microvascular Endothelial cells: Large Nerve Histology Axon loss Varied: Toluidine blue Large > Small |
![]() C5b-9 stain HIEM Nerve C5b-9 Stains Most Endoneurial Microvessels |
Disease Control Nerve C5b-9 Stains Perineurium & Epineurial vessels but not Endoneurial microvessels ![]() C5b-9 stain |
![]() C5b-9 stain |
![]() C5b-9 stain |
Endoneurial Microvasculopathy HIEM: C5b-9 stains Endoneurial microvessels ![]() C5b-9 stain |
Disease Control Nerve C5b-9 does not stain Endoneurial microvessels ![]() C5b-9 stain |
![]() C5b-9 (Green) + UEAI (Red) stain |
![]() C5b-9 (Green) + UEAI (Red) stain |
![]() C5b-9 (Green) + UEAI (Red) stain |
![]() Toluidine blue stain |
![]() Toluidine blue stain |
![]() Toluidine blue stain From R Schmidt |
![]() H&E stain HIEM nerve: No lymphocytic inflammation |
![]() VvG stain |
![]() ATPase pH 4.3 stain |
![]() Toluidine blue stain |
![]() Toluidine blue stain |
![]() Toluidine blue stain |
![]() Neurofilament stain |
![]() Neurofilament stain |
Microvasculopathy![]() |
Control Muscle![]() |
![]() |