Age changes Pi granules Blood-Nerve barrier Elzholz body Endoneurium Epineurium Perineurium Morphology Sural nerve Adults Axons Unmyelinated Myelinated Schmidt-Lanterman Cleft Morphology Node of Raniver Ultrastructure Epineurium Perineurium Schwann cells Vessels Endoneurial Epineurial Children Infraorbital nerve Sciatic nerve (Adult) Myelin Sciatic Sural Mouse |
![]() NCAM stain Non-Myelinating (Remak) Schwann cells in clusters (NCAM stain)
Mild staining is also present in some myelin sheaths |
Perineurium
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Structure Fascicles |
![]() H&E stain Normal endoneurium, perineurium, epineurium & vessels |
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Nerve fascicles Contain normal numbers of myelinated axons in endoneurium ![]() VvG stain |
![]() Gomori trichrome stain |
![]() VvG stain |
![]() H&E stain |
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![]() Neurofilament stain |
![]() Toluidine blue stain |
![]() Toluidine Blue stained plastic section |
![]() Electron micrograph (Robert E Schmidt MD) |
![]() Electron micrographs (Robert E Schmidt MD) Unmyelinated axons Surrounded by Schwann cells |
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![]() NCAM1 stain |
![]() NCAM stain |
![]() Neurofilament stain (Green) NCAM stain (Red) Overlap (Yellow) |
![]() NCAM stain (Green) p0 stain (Red) |
![]() NCAM stain (Green) MBP stain (Red) Epineurial vessels & Supporting cells in endoneurium
Epineurial Vessels
Artery (Black arrow): Linear fibrils near lumen; Thick wall Vein (White arrow): Interlaced, thin fibrils around outside of vessel; Thin wall
Schmidt-Lanterman Cleft (Incisure)
Pi (Reich) Granules
Mast cell
Myelin & Schwann cells: Sural nerveP0 (Red) Prominent in myelin around most (large & small) myelinated axons Smallest axons (Green), often in clusters, have no myelin or associated P0 staining
Myelin basic protein (MBP) in Myelin (Red) Most prominent in myelin around largest myelinated axons In myelin around fewer axons than P0 (Above)
Non-myelinating Schwann cells Nerve: Sciatic
Non-myelinating Schwann cells
Myelin Components
Prominent in myelin around most (large & small) myelinated axons Smallest axons (Green), often in clusters, have no myelin or associated P0 staining
Myelin basic protein (MBP) (Red) Most prominent in myelin around largest myelinated axons
P0 vs Myelin Basic Protein (MBP) in Myelin
Associated with large & small axons More around periphery of myelin sheath Myelin basic protein Mainly associated with largest axons More toward inside of myelin sheath
Nerve: Infraorbital (Branch of Trigeminal)
Acid phosphatase positive cells scattered in endoneurium (Above) Alkaline phosphatase positive vessels: Few in endoneurium (Below)
ATPase activity: Abundant in endoneurial cells
Infraorbital Nerve Larger myelinated axons: Many Small unmyelinated axons: Fewer than in sural nerve
Non-myelinating (Remak) Schwann cells : Fewer than in sural nerve
Non-myelinating (Remak) Schwann cells Myelinating Schwann cells: Adaxonal & Abaxonal cytoplasm
Infraorbital Nerve Larger myelinated axons (Green): No associated NCAM; Many Small unmyelinated axons (Yellow): Fewer than in sural nerve
Infraorbital Nerve: P0 (Red) Prominent in myelin (Red) around most (large & small) myelinated axons Smallest axons (Green) have no myelin or associated P0 staining
Infraorbital Nerve Myelin basic protein (MBP) is markedly reduced in myelin around axons compared to sural nerve
Nerve: Infant
Axons are mildly small Myelin sheaths around large axons are moderately thin Compare to: Adult
Normal nerve: MouseAxon populations: 3Myelinated, Large Myelinated, Small Unmyelinated
Return to Biopsy illustrations Return to Neuromuscular Home Page Return to Nerve biopsy References 1. Exp Neurol 2020 Mar 3;328:113272 3/15/2023 |