Proliferation of Remak cells: Response to degeneration of myelinated axons
Büngner Bands
Toluidine blue
Bands of Büngner: Collections of processes from Denervated Schwann Cells
Denervated Schwann cells: Have normal Basal lamina outside surface membrane
Büngner Band Cells: Schwann cells with No associated axon
Small & Large Axon loss: Severe
Neurofilaments (Green); P0 (Red)
Büngner Band cells stain for P0 protein (Red; In absence of myelin) & NCAM Axon loss: Little staining for large or small axons (Green) Büngner bands
p0 & NCAM co-localize in Schwann cells with no associated axons (Yellow)
Small NCAM positive cells (Green) are associuated with remaining small, unmyelinated axons Normal: Many p0 positive myelin sheaths with no NCAM
In nerves with axon loss but no Büngner bands, remaining denervated Schwann cells express NCAM but not P0.
NCAM (Green) & p0 (Red) stains
Loss of all myelinated axons
VvG stain
Loss of all axons
Neurofilament + Gomori stain
Non-Myelinating Schwann cells (NCAM stain): Remain present all through endoneurium
