CIDP: Nerve morphology
H&E stain
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Endoneurium
Some regions are pale
Myelinated axons: Patchy loss
Perineurium: Thin & pale stained in regions
Gomori trichrome stain
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Onion bulbs, Small (Arrows)
Small & Varied sizes
Scattered in endoneurium
Gomori trichrome stain
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Myelinated axons: Patchy, severe loss
Onion bulbs, Small
Scattered in endoneurium
VvG stain
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CIDP
Myelinated axon loss
There are no large myelinated axons
Remaining small axons are hypomyelinated
Subperineurial edema: Narrow; Surrounds much of fascicle
Toluidine blue stain
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CIDP: Loss of the largest myelinated axons
Myelin containing MBP is mostly lost
With loss of large axons many non-myelinating Schwann cells express small amounts of MBP_
Neurofilaments (Green) + MBP (Red) stain
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Myelin Pathology
Onion bulbs
Myelin sheath: Thin
Thinly myelinated axons.
Axon loss.
|
Thinly myelinated axons.
Axon loss: Patchy
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Onion bulbs: Small
|
Neurofilaments (Green) + NCAM (Red) stain
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Onion bulbs: Many small
Most axons, even intermediate sized ones, are surounded by NCAM-stained Schwann cells
NCAM normally surround only the smallest axons
Axon loss: The largest axons are completel lost
Neurofilaments (Green) + NCAM (Red) stain
|
Onion bulbs: Many small
Many axons are surrounded by P0 even though myelinated axons are mostly lost
Schwann cells in onion bulbs: Similar to Büngner band Schwann cells (Yellow) with co-staining for NCAM & P0
NCAM (Green) + P0 (Red) stain
|
Toluidine blue stain
|
Onion bulbs
Surround some axons
Varied sizes
Some contain no axon
Most axons in onion bulbs are hypomyelinated (Thin myelin sheath)
Toluidine blue stain
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CIDP: Axon loss, Chronic: Schwann cells
Büngner band & Onion bulb Schwann cells (Yellow) co-stain for NCAM & P0
Normally unmyelinated axons (Green): Occur in small clusters
NCAM (Green) + P0 (Red) stain
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Subperineurial edema
H&E stain
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Subperineurial edema
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Toluidine blue stain
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Subperineurial edema: Mild
Onion bulbs
Endoneurial Microvessels: Mildly large
Toluidine blue stain
|
CIDP, Chronic: No inflammation
Acid phosphatase stain
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CIDP: Muscle
ATPase pH 9.4 stain
Type 1 muscle fiber predominance
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Active demyelination
4/16/2021