Vacuoles No vacuoles Rhabdomyolysis Muscle fibers: Dark necrosis Post-Rhabdomyolysis Ultrastructure |
![]() H&E stain |
Subsarcolemmal cytoplasmic vacuoles (Blebs) Most contain diffuse pale-staining material May contain myonuclei ![]() H & E stain |
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![]() VvG stain |
![]() Gomori trichrome stain |
![]() NADH stain |
Subsarcolemmal vacuoles![]() NADH stain |
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![]() NADH stain |
![]() PAS stain |
![]() Phosphorylase stain |
![]() Phosphorylase stain |
![]() PAS stain |
![]() H&E stain Linear "cracks" in muscle fibers |
![]() NADH stain Linearized pattern of internal architecture: Type 2 > Type 1 |
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![]() VvG stain |
![]() Phosphorylase stain From: S Moore |
Phosphorylase stains |
![]() McArdle disease Some phosphorylase staining is present in vessels & regenerating muscle fibers |
![]() Phosphorylase stain: Normal Type II fibers: Darker than type I |
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![]() Phosphorylase stain: McArdle disease Myophosphorylase deficiency Muscle fibers stain yellow |
![]() PAS stain: McArdle disease |
![]() PAS stain: McArdle disease |
Glycogen: PAS stain Increased: Diffusely in cytoplasm Slightly metachromatic blue color in some areas May also stain contents of blebs |
![]() PAS stain: McArdle's disease |
![]() PAS stain: McArdle's disease |
Fiber types Vacuoles: May be in type I or type II muscle fibers ![]() ATPase pH 9.4 stain |
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![]() ATPase pH 4.3 stain |
![]() ATPase pH 4.3 stain |
![]() H&E stain |
![]() Gomori trichrome stain |
![]() NADH stain |
![]() H&E stain |
![]() Gomori trichrome stain |
![]() Gomori trichrome stain |
![]() NADH stain |
![]() NADH stain |
![]() PAS stain |
![]() Acid phosphatase stain |
![]() Acid phosphatase stain |
![]() Alkaline phosphatase stain |
![]() ATPase pH 4.3 stain |
![]() Phosphorylase stain |
![]() Phosphorylase stain |
![]() Gomori Trichrome stain |
![]() Alkaline phosphatase stain |
![]() H&E stain |
![]() H&E stain |
![]() Gomori Trichrome stain |
![]() Gomori Trichrome stain |
![]() VvG stain |
![]() VvG stain |
![]() Acid Phosphatase stain |
![]() Acid Phosphatase stain |
![]() NADH stain |
![]() NADH stain |
![]() H&E stain |
![]() Gomori trichrome stain |
![]() H&E stain |
![]() H&E stain |
![]() H&E stain |
![]() NADH stain |
![]() Acid phosphatase stain |
![]() PAS stain |
![]() ATPase pH 4.3 stain |
![]() Phosphorylase stain |
![]() Toluidine blue-stained plastic section From: Mari Perez-Rosendahl |
![]() From: S Moore |
![]() From: Chunyu Cai |
![]() From: Chunyu Cai |
![]() From: Chunyu Cai |
![]() H&E stain |