MYOSITIS WITH ANTI-Jo-1 ANTIBODIES

PROMINENT PERIMYSIAL PATHOLOGY H&E stain Perimysial fragmentation The perimysiun appears loculated with many focal clear areas Cells in the perimysium are most frequently macrophages. Features include Large nuclei Prominent cytoplasm Staining for Acid Phosphatase, Esterase and CD-68 Adjacent muscle fibers may be normal or show perifascicular myopathy and atrophy
H&E stain Perimysial inflammation	Acid phosphatase Perimysial cells: Acid phosphatase positive
Esterase Perimysial inflammation Esterase positive macrophages	Acid phosphatase stain CD-68 positive macrophages in perimysium
Alkaline phosphatase stain Alkaline phosphatase staining of perimysium
H&E stain Perifascicular myopathy & atrophy	Immunocytochemistry N-CAM positive atrophic muscle fibers