| |
H & E stain |
|
| Subsarcolemmal vacuoles | |
| Phosphorylase stains |
 McArdle's disease Some phosphorylase staining is present in vessels & regenerating muscle fibers |
 Phosphorylase stain: Normal Type II fibers stain more darkly than type I |
|
 Phosphorylase stain: McArdle's disease Myophosphorylase deficiency Muscle fibers stain yellow |
|
| NADH stains | |
 NADH stain Linearized internal architecture In type II (paler) muscle fibers. Occurs in ~50% of biopsies. |
|
 Subsarcolemmal bleb Longitudinal section |
|
PAS stain Increased glycogen Glycogen is increased all through cytoplasm. Type II fibers stain more darkly than type I. |
ATPase pH 9.4 stain Vacuoles are present in both type I and type II muscle fibers |