 H & E stain |
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Bimodal distribution of fiber size Larger fibers are hypertrophied and slightly paler than normal sized fibers |
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 Gomori trichrome stain |
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Bimodal distribution of fiber size Larger fibers are hypertrophied and slightly paler than normal sized fibers Some larger muscle fibers with intermediate degrees of sarcoplasmic staining are present |
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 ATPase pH 9.4 |
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Fiber types Larger, hypertrophied fibers are Type 2 (Dark) Smaller fibers are Type 1 (Light) |
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 ATPase pH 4.3 |
 ATPase pH 4.6 |
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Fiber types Larger, hypertrophied fibers are Type 2 (Light) Smaller fibers are Type 1 (Dark) Type 2C fibers (Intermediate) are absent |
Fiber types Some larger, hypertrophied fibers are Type 2A (Very Dark) Some larger, hypertrophied fibers are Type 2B (Intermediate) The degree of staining of 2B fibers is unusually variable Smaller fibers are Type 1 (Light) |
 Cytochrome oxidase stain |
 SDH stain |
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Mitochondrial stains Larger, hypertrophied type 2 fibers are Light Smaller, type 1 fibers are dark An unusual 3rd population of hypertrophied fibers with intermediate staining is present |
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 PAS stain |