TUBULAR AGGREGATES

H & E stain
Pathology Cytoplasmic H & E Internal slits or lakes May be clear, or contain pink material Gomori trichrome Contain red material Location: Central or sub-sarcolemmal VvG stain: Gray NADH-TR reductase: Stain strongly Often in type II &#gt; I muscle fibers No reaction with mitochondrial stains Ultrastructure (below): 3 types Differential Diagnosis

	Gomori trichrome stain Tubular aggregates stain red

VvG stain Tubular aggregates stain gray-black

NADH-TR reductase (NADH) stain Tubular aggregates stain dark

Tubular aggregates: In some syndromes more commonly occur (Pale regions) in type 2 (Dark) muscle fibers ATPase pH 9.4 stain

Tubular aggregates: Stain for lipid on Sudan black Sudan black stain

Tubular aggregates: Ultrastructure; 3 types

• I: 50 to 70 nm diameter tubules with 40 nm inner central tubule
• II: 70 to 400 nm tubules with moderately dense material in center
• III: 130 to 400 nm diameter tubules containing several 25 to 40 nm tubules

From T Mozaffar