Trunk Muscles

H&E stain
|
Fiber size: Mild variation
Endomysial connective tissue: Normal
Perimysial Vessels: Normal

H&E stain
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Congo red stain
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Nuclei: Normally present at periphery of muscle fibers

Gomori trichrome stain
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Internal architecture: Mildy prominent or coarse

VvG stain
|
Internal architecture

NADH stain
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Internal architecture
Varied degrees of coarseness & irregularity
"Alveolar" fibers: Similar to those seen in CACNA1S congenital myopathy

NADH stain
|
Some muscle fibers have
Internal clear regions
Irregular internal architecture

NADH stain
|

ATPase pH 9.4 stain
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Fiber types
Some fascicles, or entire muscles, can have marked type I fiber predominance
Fiber type proportions can vary among fascicles

ATPase pH 9.4 stain
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No fiber type grouping

ATPase pH 9.4 stain
|

Alkaline phosphatase stain
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Endomysial capillaries
Some, more than in limb muscles, stain for alkaline phosphatase

Alkaline phosphatase stain
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Muscle: Other Structures
Muscle structures
Vessels
Arteries: Fibrillar internal layer
Veins: (Black arrows): Wall may have no fibrils (Above) or many curved fibrils (Below)
Endomysial capillaries (White arrow)
Spindle (Contains small round muscle fibers)
Intramuscular nerve (Myelinated axons are dark-stained)
Perimysium: Connective tissue between fasciclas (Pink-stained): Contains irregular fibrils
Vascular perimysium: Contains intermediate-sized arteries & veins + lymphatics
Avascular perimysium: Contains no vessels
Avascular Perimysium
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5/10/2020