DM1 DM2 |
Chronic Late Mild |
![]() Rossolimo "De la myotonie atrophique" 1902 |
Muscle fibers Size: Variation Myonuclei Numbers: Increased Shapes: Irregular Internal Present in many muscle fibers Several in individual fibers Often clumped & in longitudinal chains Pyknotic clumps Acid phosphatase + granules Replacement by Fat ![]() H&E stain |
Perimysium: Connective tissue replaced by fat![]() H&E stain |
![]() Congo red stain |
![]() Congo red stain |
![]() H&E stain |
Internal nuclei: Many; Irregular shapes; Often in longitudinal chains![]() H&E stain |
![]() H&E stain |
![]() Gomori trichrome stain Internal nuclei Nuclei may be mildly large & clustered |
![]() Rossolimo "De la myotonie atrophique" 1902 |
![]() Gomori trichrome stain |
Internal architecture of muscle fibers: Disordered Aggregates Clear regions ![]() NADH stain |
![]() NADH stain |
Aggregates in muscle fiber cytoplasm![]() AMPDA stain |
Fiber type abnormality: All fibers are type 1![]() ATPase pH 9.4 stain |
Acid phosphatase positive granules in muscle fibers
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![]() Acid phosphatase stain |
![]() Acid phosphatase stain |
![]() H&E stain |
![]() Gomori trichrome stain |
![]() VvG stain |
![]() H&E stain |
![]() Congo red stain |
![]() H&E stain |
![]() H&E stain |
![]() H&E stain |
![]() H&E stain Fiber sizes: Internal nuclei: In some fibers |
![]() NADH stain Type I (darker) fibers: Smaller Internal architecture: Disordered |
![]() NADH stain |
![]() ATPase pH 9.4 stain |
![]() Emerin stain |
![]() Emerin stain |
![]() H&E stain Muscle fibers Size: Atrophy & Some hypertrophy Pyknotic nuclear clumps Nuclei: Large & Irregular shapes Endomysial connective tissue: Moderately increased |
![]() Congo red stain |
![]() VvG stain |
![]() H&E stain |
![]() H&E stain |
![]() H & E stain |
![]() H & E stain |
![]() H & E stain |
![]() Congo red stain |
![]() H & E stain Internal nuclei: Multiple in individual fibers |
![]() ATPase pH 9.4 Internal nuclei (clear regions) Most commonly in type 2 (dark) muscle fibers |
![]() ATPase pH 9.4 |
![]() ATPase pH 4.6 |
![]() ATPase pH 4.6 |
![]() ATPase pH 4.3 |
![]() ATPase pH 4.3 |
LYSOSOMAL Structures Cytoplasmic granules: Acid Phosphatase positive granules: Scattered in cytoplasm ![]() Acid Phosphatase |
![]() Acid Phosphatase |
![]() H & E stain |
![]() H & E stain |
![]() Congo red stain |
![]() Congo red stain |
![]() H & E stain |
![]() NADH stain |
![]() Esterase |
Pyknotic nuclear clumps: Dark-stained
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![]() NADH stain |
![]() From: Cory Toth MD Pyknotic nuclear clump: Ultrastructure
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![]() Congo red stain |
![]() Emerin stain |
![]() Sudan Black stain |