H&E stain

H&E stain
Muscle fibers: Varied sizes
Muscle fibers: Vacuoles, irregular shaped; Large nuclei

VvG stain
Vacuoles in muscle fibers

VvG stain

NADH stain
Internal architecture
  Linearization in pale stained fibers

Vacuoles: Contain
  Basophilic granular debris
  Red-green birefringent material

Congo red stain

Congo red stain

Congo red stain

Vacuoles: Associated with AMPDA positive agggregates

AMPDA stain
Acid phosphatase positive material: Some associated with vacuoles

Acid phosphatase stain
Immature muscle fibers

ATPase pH 4.3 stain

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