H & E stain |
Myopathy: Mild active & chronic changes Necrosis & Regeneration: Scattered Fiber size: Varied Small fibers: Rounded or Polygonal Endomysial connective tissue: Normal or Slightly increased Internal nuclei: Some fibers |
H & E stain |
Myopathy Necrosis: Scattered Fiber size: Varied Internal nuclei: Some fibers Gomori trichrome stain |
VvG stain |
Myopathy Small muscle fibers: Both types; Scattered 2C muscle fibers: Few ATPase pH 9.4 stain |
ATPase pH 4.3 stain |
Small muscle fiber: : Regenerating, Coarse internal architecture NADH stain |
Necrotic muscle fiber: Replaced by esterase+ cells Esterase stain |
Aggregated Caveolin-3: Sub-sarcolemmal Caveolin-3 stain |
α-Dystroglycan (Green): Reduced or Absent on the surface of some muscle fibers α-Dystroglycan - Green; Caveolin-3 - Red |