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RFC1: CANVAS

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H&E stain

RFC1: Sural nerve
  Normal structure of connective tissue & vessels

H&E stain

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VvG stain

Gomori trichrome stain

Myelinated Axons
  Reduced numbers

VvG stain

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Endomysium
  Normal ATPase staining

ATPase pH 4.3 stain

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Alkaline phosphatase stain

Endoneurial Microvessels
  Numbers: Normal to Increased
  Alkaline phosphatase staining: Reduced

UEAI stain

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VvG stain

Epineurial Arteries
  Irregular inner fibril layer

VvG stain

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Congo red stain

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No endoneurial histiocytes

Acid phosphatase stain

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Neurofilament stain

Axons
  Large: Severe loss
  Small, Unmyelinated: Relatively preserved

Neurofilament stain

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Neurofilament (Green)-P0 (Red) stain

Axons
  Large: Severe loss

Neurofilament (Green)-MBP (Red) stain

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Neurofilament (Green)-NCAM (Red) stain

Axons
  Small: Mild loss; Some non-myelinating Schwann cells have no axons (Red)

NCAM stain

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NCAM (Green) - P0 (Red) stain

Schwann cell pathology: Chronic
  Büngner band cells (Yellow) present

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Schwann cell pathology: Chronic
  Büngner band cells (Red) present
  Myelinated axons (Yellow): Reduced numbers

Periaxin (Green) - P0 (Red) stain

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RFC1: Axons

Toluidine blue-stained Plastic sections

Axon loss
  Large & Small myelinated axons
Axon regeneration
  Clusters of small, thinly myelinated axons
Axon shapes
  Many irregularly shaped small myelinated axons

Toluidine blue-stained Plastic sections

Toluidine blue-stained Plastic sections

Axon loss
  Large & Small myelinated axons
Axon regeneration
  Clusters of small, thinly myelinated axons
Axon shapes
  Many irregularly shaped small myelinated axons

Toluidine blue-stained Plastic sections

Toluidine blue-stained Plastic sections

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3/22/2022