RFC1: CANVAS
![](../pics/biopsy/nerve/rfc1/rfc1help.jpg)
H&E stain
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RFC1: Sural nerve
Normal structure of connective tissue & vessels
![](../pics/biopsy/nerve/rfc1/rfc1hemp2.jpg)
H&E stain
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![](../pics/biopsy/nerve/rfc1/rfc1vvg.jpg)
VvG stain
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![](../pics/biopsy/nerve/rfc1/rfc1axlossgtmp.jpg)
Gomori trichrome stain
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Myelinated Axons
Reduced numbers
![](../pics/biopsy/nerve/rfc1/rfc1axlossvvghp.jpg)
VvG stain
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Endomysium
Normal ATPase staining
![](../pics/biopsy/nerve/rfc1/rfc1atp43.jpg)
ATPase pH 4.3 stain
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![](../pics/biopsy/nerve/rfc1/rfc1alp.jpg)
Alkaline phosphatase stain
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Endoneurial Microvessels
Numbers: Normal to Increased
Alkaline phosphatase staining: Reduced
![](../pics/biopsy/nerve/rfc1/rfc1ulexmp.jpg)
UEAI stain
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![](../pics/biopsy/nerve/rfc1/rfc1vessvvghp.jpg)
VvG stain
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Epineurial Arteries
Irregular inner fibril layer
![](../pics/biopsy/nerve/rfc1/rfc1smvessvvg.jpg)
VvG stain
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![](../pics/biopsy/nerve/rfc1/rfc1cr.jpg)
Congo red stain
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No endoneurial histiocytes
![](../pics/biopsy/nerve/rfc1/rfc1acp.jpg)
Acid phosphatase stain
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![](../pics/biopsy/nerve/rfc1/rfc1nfil.jpg)
Neurofilament stain
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Axons
Large: Severe loss
Small, Unmyelinated: Relatively preserved
![](../pics/biopsy/nerve/rfc1/rfc1nfil2.jpg)
Neurofilament stain
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![](../pics/biopsy/nerve/rfc1/rfc1nfp0.jpg)
Neurofilament (Green)-P0 (Red) stain
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Axons
Large: Severe loss
![](../pics/biopsy/nerve/rfc1/rfc1nfmbp.jpg)
Neurofilament (Green)-MBP (Red) stain
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![](../pics/biopsy/nerve/rfc1/rfc1nfncam.jpg)
Neurofilament (Green)-NCAM (Red) stain
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Axons
Small: Mild loss; Some non-myelinating Schwann cells have no axons (Red)
![](../pics/biopsy/nerve/rfc1/rfc1ncam.jpg)
NCAM stain
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![](../pics/biopsy/nerve/rfc1/rfc1ncamp0.jpg)
NCAM (Green) - P0 (Red) stain
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Schwann cell pathology: Chronic
Büngner band cells (Yellow) present
Schwann cell pathology: Chronic
Büngner band cells (Red) present
Myelinated axons (Yellow): Reduced numbers
![](../pics/biopsy/nerve/rfc1/rfc1prxgp0r.jpg)
Periaxin (Green) - P0 (Red) stain
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RFC1: Axons
![](../pics/biopsy/nerve/rfc1/rfc1-1.jpg)
Toluidine blue-stained Plastic sections
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Axon loss
Large & Small myelinated axons
Axon regeneration
Clusters of small, thinly myelinated axons
Axon shapes
Many irregularly shaped small myelinated axons
![](../pics/biopsy/nerve/rfc1/rfc1-2.jpg)
Toluidine blue-stained Plastic sections
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![](../pics/biopsy/nerve/rfc1/rfc1-3.jpg)
Toluidine blue-stained Plastic sections
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Axon loss
Large & Small myelinated axons
Axon regeneration
Clusters of small, thinly myelinated axons
Axon shapes
Many irregularly shaped small myelinated axons
![](../pics/biopsy/nerve/rfc1/rfc1-4.jpg)
Toluidine blue-stained Plastic sections
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![](../pics/biopsy/nerve/rfc1/rfc1-5.jpg)
Toluidine blue-stained Plastic sections
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RFC1
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Neuromuscular Home Page
3/22/2022