H & E stain
Calcification of perimysial vessel walls

Alizarin red stain
Calcium deposited in vessel walls (Staining with alizarin red)

Variable fiber size Type 2 atrophy
Muscle pathology in caliciphylaxis
  • Variability in muscle fiber size
  • Type 2 muscle fiber atrophy
  • Muscle fiber necrosis (Not shown)

Alkaline phosphatase Hemosiderin
Subcutaneous tissue pathology in caliciphylaxis
  • Alkaline phosphatase staining
  • Hemosiderin deposits

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