Neuromuscular

DISTAL MYOPATHY: SQSTM1


H&E stain
Chronic myopathy
Fiber size: Varied from Small to Large, Occasional pyknotic nuclear clumps
Perimysium & Endomysium: Replaced by fat
No necrosis or regeneration

H&E stain


H&E stain
Small muscle fibers
Some have basophilic cytoplasm & large nuclei
Irregular internal architecture or Dark staining

NADH stain

VvG stain

Vacuoles

VvG stain
Vacuoles: Black-stained rim on VvG

VvG stain

VvG stain

Gomori trichrome stain

Gomori trichrome stain
Vacuoles: Red-stained rim on GT

Gomori trichrome stain

Gomori trichrome stain
Vacuoles: Red staining (? amyloid-like) on Congo red

Congo red stain

Congo red stain
Vacuoles: AMPDA granules

AMPDA stain

Fiber types

ATPase pH 9.4 stain
Abnormal fiber types
Intermediate-stained muscle fibers at ATPase pH 4.3

ATPase pH 4.3 stain

MHC Class I: Upregulated on muscle fiber surface

MHC Class I stain


Esterase stain
Neuromuscular junctions

Esterase stain

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7/31/2015