 H&E stain |
 H&E stain |
 H&E stain |
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Varied muscle fiber size: Atrophy & Hypertrophy Regenerating (Basophilic) muscle fibers Endomysial connective tissue: Moderately increased Inflammation: Around intermediate sized vessel | ||
 H&E stain |
 H&E stain |
 Acid phosphatase stain |
| Necrotic muscle fibers: Scattered; More common in muscles with less fibrosis | ||
 ATPase, pH 4.3 stain Small & large fibers are both types.
A few immature, type 2C, fibers are present. (Intermediate staining; Arrow) |
 Sudan black stain Lipid droplets can accumulate in the perimysium. |
 H&E stain FSH fibers
Basophilic muscle fibers with clustered large nuclei |
 MHC-1 stain MHC-1 is upregulated on the rim of all muscle fibers and normally present in the cytoplasm of small immature fibers. |
 H&E stain More marked variation in fiber size
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 NADH stain Hypertrophied fibers have pale centers.
Some small fibers are darkly stained |
 ATPase pH 9.4 stain, Patient 1 Large fibers are type I & II.
Small fibers are type I & II. |
 ATPase pH 9.4 stain, Patient 2 Large fibers are type I & II.
Small fibers are often type II. |
 H&E stain Varied muscle fiber size
Many small basophilic fibers Mononuclear inflammatory cells around intermediate sized perimysial vessel |
 H&E stain |
 ATPase pH 9.4 stain |
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Variable muscle fiber size. Basophilic (immature) fibers. No hypertrophy. Endomysial inflammation. |
Small fibers are Type I & II
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 MHC-1 stain MHC-1: Abnormal upregulation on muscle fiber surfaces
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