Neuromuscular

Cytoplasmic Bodies



Gomori Trichrome stain
Cytoplasmic bodies
  • Structure
    • Central region: Homogenous; Dark stain on Gomori trichrome similar to rods
    • Outer zone: Radially oriented filaments; May contain actin
  • Location
    • Within cytoplasm: Central or subsarcolemmal
    • Early stage: May be associated with Z-Band
  • Histochemical properties
    • Stain strongly with Gomori trichrome & Myoadenylate deaminase
    • Stain poorly with myofibrillary ATPase & NADH
    • Type I fibers in distal type; Type I or II fibers in limb-girdle syndromes
    • PAS +
    • Molecular contents
      • General: Actin; Positive staining for phalloidin
      • Other variable: Desmin, Dystrophin, β-Amyloid precursor, N-CAM
    • Differential diagnosis: Nemaline rods
  • Ultrastructure: Variable
    • Foci of myofibrillary destruction: Z-disk derived bodies & streaming
    • Cytoplasmic or spheroid bodies: Thick & thin filament remnants
    • Aggregates of Z-disk filaments
  • Disease associations


Gomori trichrome

Gomori trichrome

Gomori trichrome

Gomori trichrome

Phalloidin for actin

From Robert Schmidt MD

Toluidine blue stain

 

 

From Robert Schmidt MD

Ultrastructure

 

Return to Neuromuscular Home Page
Return to Pathology index

12/3/2014